With more than 800 members, G protein-coupled receptors (GPCRs) constitute the largest family of membrane receptors. They are central players in response to a wide variety of extracellular stimuli and mediate diverse cellular responses. GPCRs are implicated in the pathogenesis of numerous diseases and are of particular importance for drug discovery.
As integral membrane proteins, GPCRs are generally unstable when removed from their membrane environment, which makes it difficult to apply a range of biophysical and structural techniques for soluble proteins to GPCRs. After solubilization, GPCRs require robust purification protocol prior to further studies. Therefore, optimization of purification remains a matter of trail and error. During the entire purification process, it is most important to ensure that experimental conditions maintain the active state of the GPCR. General affinity tags, receptor-specific ligand columns, and size exclusion chromatography are commonly used for GPCR purification.
CD BioSciences has built an unparalleled platform for GPCR purification to provide the most reliable service for research projects. Whether researchers require labeled or untagged GPCRs, single proteins or GPCR complexes, small-scale or large-scale purification, CD BioSciences' skilled and experienced scientists can develop the best strategy to meet their specific requirements.
CD BioSciences is ready to provide a series of GPCR purification services to its customers, for instance, the Affinity Chromatography Service. Affinity chromatography is commonly used as the first chromatography step in GPCR purification. Recombinant cloning technology makes it easy to introduce universal affinity tags at the N- or C-terminal of GPCRs. Receptor-specific ligand affinity chromatography may be required as a second step to isolate pure and functional GPCRs.
Ion-Exchange Chromatography Service is also offered. While it is not a general purification method, it can also be used to purify several GPCRs. For example, anion-exchange chromatography is often utilized for GPCR purification, where anionic detergents with anion-exchange columns and cationic detergents with cation-exchange columns need to be avoided.
In addition, CD BioSciences provides the evaluation of Membrane Protein Service. The purified GPCRs will be evaluated carefully using SDS-PAGE to ensure the expected properties of target proteins can be maintained. As always, this is an important step for further functional and structural studies.
CD BioSciences provides comprehensive and professional protein-based solutions for GPCR research and drug development. If you are interested in GPCR purification services or have any specific inquiries, please visit its site at https://www.progpcr.com.
about CD BioSciences
CD BioSciences is a leading customer-focused biotechnology company founded in New York. With unparalleled expertise in manufacturing, CD BioSciences is dedicated biological and chemical products and strives for solutions that can improve research outcomes and effectively elevate research success. CD BioSciences is committed to providing universities, research institutions, biotechnology, and pharmaceutical companies with a wide range of high-quality and reliable products, as well as comprehensive service packages related to GPCRs that will enable further advancement of basic science and drug development.
